Publications

Refereed journal articles

  1. Thole V, Worland B, Wright J, Bevan MW, Vain P (2010) Distribution and characterization of more than 1000 T-DNA tags in the genome of Brachypodium distachyon community standard line Bd21. Plant Biotechnology Journal 8: 734–747
  2. Thole V, Alves SC, Worland B, Bevan MW, Vain P (2009) A protocol for efficiently retrieving and characterising Flanking Sequence Tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants. Nature Protocols. 4:650-661.
  3. Alves SC, Worland B, Thole V, Snape JW, Bevan MW, Vain P (2009) A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21. Nature Protocols. 4:638-649.
  4. Opanowicz M, Vain P, Parker D, Draper J and Doonan J (2008) Brachypodium distachyon: making hay with a wild grass. Trends in Plant Science 13:172-177.
  5. Vain P, Worland B, Thole V, McKenzie N,  Alves SC, Opanowicz M, Fish LJ, Bevan MW and  Snape JW (2008) Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis. Plant Biotechnology Journal  6:236-245.

Poster presentation at conferences

  1. Schieszl K, Thole V, Worland B, and Vain P (2010) The pBrachyTRAP Vectors: New Tools for Promoter Trapping in Brachypodium Distachyon. Eucarpia – Cereals section meeting. Cambridge, UK, 6-8 April. P30.pdf document
  2. Thole V., Worland B., Wright J., Bevan M. W., Vain P. (2010) The pBrachyTAG vector system: New tools for Brachypodium functional genomics. Plant & Animal Genomes Conference XVIII, San Diego, USA January 9th-13h 2010, P333 pdf document
  3. Thole, V., Worland, B., Wright, J., Bevan, M.W. and Vain, P. (2009) BrachyTAG: T-DNA insertional mutagenesis in Brachypodium distachyon (genotype Bd21). 8th Plant Genomics European Meetings, Lisbon, Portugal, 7th-10th October 2009. S3-P01. pdf document
  4. Thole V, Worland B, Bevan MW and Vain P (2009) An efficient method to retrieve and characterise Flanking Sequence Tags (FSTs) in Brachypodium distachyon T-DNA insertional mutants.  Plant & Animal Genomes Conference XVII, January 10-14, San Diego, USA. Poster 249. PDF document
  5. Alves SC, Worland B, Thole V, Bevan MW, Snape JW and Vain P (2008) High throughput Agrobacterium-mediated transformation of Brachypodium distachyon (Line Bd21) for functional genomics. Plant & Animal Genomes Conference XVI, January 12-16, San Diego, USA. Poster 668. PDF document
  6. Alves SC, Worland B, Thole V, Bevan MW, Snape JW and Vain P (2007) High throughput Agrobacterium-mediated transformation of Brachypodium distachyon (Line Bd21) for functional genomics. Garnet Conference, September 10-11, Norwich, UK. Poster 37. PDF document
  7. Vain P, Worland B, Thole V, Fish L, Bevan MW and Snape JW (2007) Transformation of the Temperate Grass Brachypodium Distachyon (Line Bd21) Using the Green Fluorescent Protein (GFP) as a Screenable Marker. Plant & Animal Genomes XV Conference, January 13-17, San Diego, USA. Poster 704. PDF document

Talks at conferences

  1. Vain P (2010) BrachyTAG: T-DNA Insertional Mutagenesis in Brachypodium Distachyon (Genotype Bd21). Eucarpia – Cereals section meeting, Cambridge, UK - Invited speaker.
  2. Vain P. (2010) "BrachyTAG: T-DNA tagging in Brachypodium distachyon (Genotype Bd21)" PAG-XVIII conference, San Diego, USA - Invited speaker
  3. Vain P. (2009) “BrachyTAG: T-DNA tagging in Brachypodium distachyon (Genotype Bd21)”. Crop Genetics Department, John Innes Centre, Norwich, UK - Seminar
  4. Vain P. (2008) “Transformation and T-DNA tagging in Brachypodium distachyon (BrachyTAG)”. Annual Science Meeting , John Innes Centre, Norwich, UK - Seminar
  5. Vain P. (2008) “Transformation and T-DNA tagging in Brachypodium distachyon (genotype Bd21)”. INRA, Versailles, France - Invited site seminar
  6. Vain P. and Vogel J. (2008) “Transformation and T-DNA tagging in Brachypodium distachyon”. PAG-XVI conference, San Diego, USA - Invited speaker
  7. Vain P. (2008) “Brachypodium transformation”. Brachypodium workshop, John Innes Centre, Norwich, UK - Seminar
  8. Bevan M., McKenzie N., Vain P., Wright J. and Snape J. (2007) Developing Brachypodium distachyon as an experimental system for temperate grasses. Garnet Conference, John Innes Centre, Norwich, UK - joint seminar